Cyclin Gl、Mdm2和P53在胃癌组织中的表达及相关性研究

The expressions and correlations of Cyclin Gl,Mdm2 and P53 in gastric cancer

目的探讨细胞周期蛋白G1(Cyclin G1)、鼠双微体基因(Mdm2)和P53在胃癌组织中的表达意义及相关性。方法采用免疫组织化学方法(SP法)检测54例胃癌组织中Cyclin G1、Mdm2和P53的表达,以20例正常胃黏膜组织作为对照。结果胃癌组织中Cyclin G1、Mdm2、P53的阳性表达率分别为62.96%、53.70%、44.44%,与正常组织对比,有显著性差异(P<0.05),Cy-clinGl、Mdm2的阳性表达率和表达强度与胃癌的分化程度相关。胃癌组织中Mdm2蛋白的表达与P53的表达呈正相关(r=0.307),而CyclinGl蛋白的表达与P53的表达无明显相关性。结论CyclinGl、Mdm2、P53的过表达在胃癌的发生、发展过程中起着重要的的作用。Mdm2可能是通过调控P53的活性而促进胃癌的发生、发展,Cyclin G1可能以不依赖P53的途径发挥作用。

Objective To study the expressions and correlations of Cyclin G1, Mdm2 and P53 in gastric carcinoma. Methods Formalin fixed, paraffin wax embedded tissues from 54 gastric carcinomas were investigated by means of immunohistochemistry for Cyclin G1, Mdm2 and P53. Results The positive rates of cyclin G1, Mdm2 and P53 protein expression in gastric carcinoma were respectively 62.96% , 53.70% and 44.44% while in the normal tissue neither the expression of Cyclin G1 nor P53 was positive, the positive rate of Mdm2 in normal tissue was 15%. there were significant difference between them （P 〈0.05）. The positive exepression rate and intensity of Cyclin G1 and Mdm2 in gastric cancinoma were significantly correlated with tumor differentiation （p 〈 0.05 ）. Moreover, The expression of Mdn12 was positively correlated with P53 protein ,and no associations were found between the expression of P53 and Cyclin G1 in gastric carcinomas. Conclusion Overexpressions of CyclinG1, Mdm2 and P53 might play important roles in the occurrence and development of gastric carcinoma.