胃癌组织中COX-2、VEGF的表达及临床意义

Espression and significance of COX-2 and VEGF in human gastric carcinoma

目的探讨COX-2和VEGF在人胃癌组织中的表达及临床意义。方法应用免疫组化SABC法检测45例人胃癌组织中COX-2、VEGF的表达,并以30例正常胃黏膜组织作为对照。对COX-2、VEGF的表达采用平均光密度测定,并对临床相关资料进行统计学分析。结果胃癌组织中COX-2、VEGF的平均光密度值(OD)为19.85±7.34,134.67±13.11,显著高于正常胃黏膜组织的表达(12.49±4.87,83.89±8.74,P<0.01),癌组织中COX-2、VEGF的表达与性别、年龄、部位、组织类型、分化程度无相关性,而与肿瘤直径、TNM分期、淋巴转移、远处转移(P<0.05)相关;同时,胃癌组织中COX-2与VEGF表达有显著相关性(r=0.473,P=0.005)。结论COX-2可能诱导VEGF的表达而促进肿瘤血管的生成和侵袭转移。

Objective To study the expression of cyclooxygenase-2（COX-2） and endothelial growth factor（VEGF） in gastric carcinoma and the possible clinic significance. Methods The expression of COX-2 and VEGF were studied by immunohistochemistry SABC method in 45 gastric carcinoma specimen and 30 normal gastric tissues as control. The averaged light density was assayed by the expression of COX-2 and VEGF, and the statistic software was used to analyze the clinic correlated information. Results The averaged light density （OD） of COX-2 and VEGF in gastric carcinoma were 19.85 ± 7.34,134.67 ± 13.11 respectively, which was significantly higher than that in the normal gastric tissues （ 12.49 ±4.87,83.89 ± 8.74,P 〈 0.01 ） o The expression of COX-2 and VEGF was associated with the diameter of tumor,TNM stage,lymph node metastasis and distant metastasis （P 〈 0.05 ） , but not with age, gender, tumor position, cellular differentiation and histological type. The expression of COX-2 was positively correlated to VEGF （r =0. 473 ,P =0. 005）. Conclusion COX-2 may induce the expression of VEGF to promote the formation of angiogenesis and the invasion and metastasis of gastric carcinoma.