VEGF-C及其受体FLT-4 mRNA的表达在胃癌淋巴转移中的作用

Effects of VEGF-C and FLT-4 mRNA expressions on lymph node metastasis in gastric cancer

目的研究血管内皮生长因子C(vascular endothelial growth factor C,VEGF-C)及其受体VEGFR-3(FLT-4)mRNA在胃癌、癌旁组织及正常组织中的表达。方法采用RT-PCR技术对34例胃癌的癌组织和癌旁组织及16例正常胃组织中VEGF-C及FLT-4 mRNA的表达进行检测。结果VEGF-C及FLT-4在胃癌和癌旁组织中都有表达;VEGF-C及FLT-4在胃癌、癌旁组织和正常胃组织中的表达有明显差异(PVEGF-C<0.05,PFLT-4<0.05);VEGF-C及FLT-4在胃癌组织中的表达有明显相关性(r=0.6729,P<0.05);有淋巴结转移的胃癌与癌旁组织中VEGF-C的表达有明显差异(r=0.5189,P<0.05);VEGF-C在中、低分化和高分化胃癌组织中的表达有明显差异(P<0.05);BorrmannⅠ、Ⅱ型分别与Ⅲ、Ⅳ型胃癌组织中VEGF-C的表达有明显差异(P<0.05),而Ⅰ、Ⅱ型之间的差异无统计学意义(P>0.05),Ⅲ、Ⅳ型之间的差异无统计学意义(P>0.05)。结论VEGF-C及其受体FLT-4在胃癌的生长中起作用;VEGF-C及其受体FLT-4 mRNA的高表达可促进胃癌淋巴管的形成和淋巴结的转移。

Objective To investigate the expression of vascular endothelial growth factor-C （VEGF-C） and its receptor VEGFR-3（FLT- 4） mRNA in primary gastric cancer, adjacent and normal tissues. Methods The expressions of VEGF-C and FLT- 4 mRNA in 34 primary gastric cancers and adjacent normal tissues from the same patients were studied by semi-quantitative reverse transcriptase-polymerase chain reaction （RT-PCR）. Results There was a significant difference in expression of VEGF-C between primary tumor and adjacent normal tissues（ PVEGF-C 〈0. 05, PFLT-4 〈 0. 05 ） , and a statistical correlation between VEGF-C and FLT-4 expression in tumors （ r = 0. 6729, P 〈 0.05）. With regard to VEGF-C expression, there was a significant difference between positive and negative lymph node metastases （r = 0. 5189, P 〈 0.05）. With regard to VEGF-C expression, there was a significant difference among Borrmann Ⅰ,Ⅱ,Ⅲ,Ⅳ （P 〈 0.05）. However, there was no significant difference between Borrmann Ⅰand Ⅱ or between Borrmann Ⅲand Ⅳ（P 〉 0.05）. Conclusion VEGF-C and its receptor FLT- 4 may play an important role in the development of gastric cancer, and the tumors with expression of VEGF-C and FLT-4 are more likely to have lymph angiogenesis and lymph node metastasis.