贲门癌组织Ras相关区域家族1A基因启动子区甲基化检测

RASSF1A gene methylation in gastric cardia adenocarcinoma tissue

目的探讨贲门腺癌(GCA)发生中Ras相关区域家族1A(RASSF1A)基因启动子区异常甲基化的作用。方法采用甲基化特异性PCR法检测81例贲门腺癌及其中20例癌旁正常组织RASSF1A基因启动子区甲基化变化特征,并分析RASSF1A基因启动子区甲基化与肿瘤大体分型、分化程度、淋巴结转移、TNM分期等临床病理特征的关系。结果GCA组织中RASSF1A基因甲基化阳性率(72%,58/81)显著高于癌旁正常组织(5%,1/20)(P<0.05)。GCA组织RASSF1A基因启动子区甲基化与肿瘤大体分型、分化程度、淋巴结转移、TNM分期等临床病理特征无关。结论RASSF1A基因甲基化是GCA癌变过程中频发的分子事件,可能在GCA发生中起一定作用。

Aim： To investigate the significance of RASSF1A gene methylation in gastric cardia adenocarcinoma （GCA）. Methods：Methylation-specific PCR was used to analyze the status of RASSF1A gene methylation in 81 cases of GCA and 20 cases of adjacent normal gastric cardiac tissue. Results： The methylation rate of RASSF1A gene in tumor tissue （72% ,58/81 ） was much higher than that （5% , 1/20） in normal adjacent cardiac tissue,P 〈 0. 05. No association was found between the RASSF1A gene methylation and tumor types, differentiation, stages, and metastasis. Conclusion： The RASSF1A methylation is a common event in GCA, which may be related with the carcinogenesis of GCA.