摘要
目的研究CYP1B1基因外显子3密码子432（C-G）多态性与子宫内膜癌易感性的关系。方法应用聚合酶链反应-限制性片断长度多态性分析（PCR-RFLP）技术检测76例子宫内膜癌患者和83例对照者的CYP1B1基因密码子432（C-G）位点多态性,并分析CYP1B1基因多态性与子宫内膜癌易感性的相关性。结果CYP1B1基因密码子432中等位基因C、G在子宫内膜癌组和对照组分布的差异有统计学意义（P〈0.05）,其中等位基因G使子宫内膜癌发病风险增加2.24倍。CYP1B1基因密码子432C/G各基因型分布两组间差异有统计学意义（P〈0.05）,纯合突变（G/G）基因型、杂合突变（C/G）基因型与野生（C/C）基因型相比,患子宫内膜癌的危险度分别提高了5.62倍和2.04倍。结论CYP1B1基因Leu432Val多态性与子宫内膜癌的发生有一定的关系,突变基因型增加子宫内膜癌的发病风险。
Objective To study the association between gene polymorphism of cytochrome P450 1B1 in exon 3 codon 432 （C - G ） and the susceptibility to endometrial cancer. Methods Polymerase chain reaction - restriction fragment length polymorphism was used to detect gene polymorphism in exon 3 codon 432（ C - G） of CYP1B1 in 76 cases of endometrial cancer and 83 cases of normal control ,and analyze the relationship between gene polymorphism of cytochrome P450 1 B1 and the susceptibility to endometrial cancer. Results The frequency of allele C, G on codon 432 C/G of CYP1B1 between the endometrial cancer group and the control group showed a siginificant difference （ P 〈 0. 05 ）, with an odds of 2.24. There was a siginificant difference in the frequency of genotype C/C, C/G, G/G between the two groups （ P 〈 0.05 ）. Compared with wild - type C/C, the susceptibility of endometrial cancer with the genotypes of homozygotic mutation G/G and heterozygote mutation C/G was increased respectively by 5.62 and 2.04. Conclusion The gene polymorphism of cytochrome P450 1B1 in exon 3 codon 432 （C -G） is concern with the susceptibility to endometrial cancer. The mutation of CYP1B1 gene increases the risk of endometrial cancer.
出处
《中原医刊》
2007年第10期9-10,共2页
Central Plains Medical Journal
