期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

RNAi及其抑制目的基因表达的机制 被引量：2

RNAi and Mechanism of Inhibition of Target Gene Expression
下载PDF
导出
摘要 系统介绍了RNAi的研究历史、作用机制、设计方法及其对目的基因表达的抑制效果、生物学意义,展望了RNAi在人类疾病预防和治疗中的应用前景。 The history of RNAi study, the mechanism, the design method, the inhibition of target gene expression and its biological significance were reviewed. The application prospect of RNAi in human disease prevention and cure was put forward.
作者 刘月英 周志平 马俊莲 张子德 唐霞
机构地区 河北经贸大学生物科学与工程学院 河北软件职业技术学院 河北农业大学食品科技学院
出处 《安徽农业科学》 CAS 北大核心 2007年第13期3826-3827,共2页 Journal of Anhui Agricultural Sciences
关键词 RNAI DSRNA SIRNA 基因沉默 RNAi dsRNA siRNA Gene silencing
分类号 Q786 [生物学—分子生物学]
  • 相关文献

参考文献13

  • 1IZANT J,WEINTRANB H.Inhibition thythidine Kinase gene expression by anti-sense RNA:a theleculat approach to genetic anaiysis[J].Cell,1984,36:1007-1015.
  • 2NAPOLI C,LEMIEUX C,JORGENSEN R.Introduction of a chalcone synthase gene into Petunia results in reversible co-suppression of homologous genens in trans[J].Plant Cell,1990,2:279-283.
  • 3COGONI C,ROMANO N,MACINO G.Suppression of gene expression by homologous transgenes[J].Antonie Van Leeuwenhoek,1994,65:206-209.
  • 4GUO S,KEMPHUES K J.Per-1,a gene required for establishing polarity in C.elegant embryos,encodes a putative Ser/Thr Kinase that is asymmetrically distributed[J].Cell,1995,81:611-620.
  • 5FIRE A.Potent and specific genetic interference by double-strand RNA in caenorhabditis elegans[J].Nature,1998,391:806-811.
  • 6马鹏鹏,薛社普,韩代书.RNA干扰技术的原理与应用[J].中国组织化学与细胞化学杂志,2003,12(2):201-207. 被引量：12
  • 7陈忠斌,于乐成,王升启.RNA干扰作用(RNAi)研究进展[J].中国生物化学与分子生物学报,2002,18(5):525-528. 被引量：35
  • 8HAMMOND S M,BERESTEIN E,BEACH D.An RNA-directed nuclease mediated genetic interference in Caenorhabditis elegans[J].Nature,2000,404:293-296.
  • 9OSHIUMI H,BEGUM N A,MATSMOTO M,et al.RNA interferferencefor mammalian cells[J].Nippon Yakurigaku Zasshi,2002,120(2):91-95.
  • 10HOLEN T,AMARZGUIOUI M,WIIGER M T,et al.Positional effects of short interfering RNAs targeting the human congulation trigger tissue facter[J].Nucleic Acids Res,2002,30(8):1757-1766.

二级参考文献59

  • 1[1]Fire A, Xu S, Montgomery M K, Kostas S A, Driver S E, Mello C C: Potent and specific genetic interference by double-strand RNA in Coenorhabditis elegans. Nature, 1998,391(6669) :744 ～ 745
  • 2[2]Hunter C P. Gene silencing: shrinking the black box of RNAi. Curr Biol, 2000, 10(4):R137～ 140
  • 3[3]Zamore P D, Tuschl T, Sharp P A, Bartel D P. RNAi: double-strand RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell, 2000,101: 25 ～ 33
  • 4[4]Caplen N J, Panish S, Imani F,Fire A,Morgan R A. Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. Proc Natl Acad Sci USA ,2001 ,98(17) :9742 ～9747
  • 5[5]Nykanen A, Haley B, Zamore P D. ATP requirements and small interferings RNA structure in the RNA interference pathway. Cell, 2001,107(3) :309 ～ 3021
  • 6[6]Matzke M,Matzke A J,Kooter J M. RNA:guiding gene silencing. Science ,2001,293(5532): 1080 ～ 1083
  • 7[7]Lipardi C, Wei Q, paterson B M. RNAi as random degradative PCR.siRNA primers convert mRNA into dsRNAs that are degraded to generate new siRNAs. Cell, 2001, 107(3):297～307
  • 8[8]Sijen T, Fleenor J, Simmer F, Thijssen K L, Parrish S, Timmons L,Plasterk R H, Fire A. On the role of RNA amplification in dsRNA-triggered gene silencing. Cell, 2001, 107(4) :465 ～ 476
  • 9[9]Nishikura K. A short primer on RNAi: RNA-directed RNA polymerase acts as a key catalyst. Cell, 2001, 107(4) :415 ～ 418
  • 10[10]Boutla A, Delidakis C, Livadaras l,Tsagris M,Tabler M. Short 5'-phosphorylated double-stranded RNAs induce RNA interference in Drosophila. Curr Biol, 2001, 11(22): 1776 ～ 1780

共引文献45

同被引文献29

引证文献2

二级引证文献1

安徽农业科学
2007年 第13期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部