皂质芦荟的组织培养研究

Study on Tissue Culture of Aloe saponaria

以皂质芦荟的叶尖、幼苗、腋芽作为外植体进行了离体培养研究。结果发现:叶尖切口处25 d左右可产生愈伤组织,再经15 d诱导可萌发再生芽;幼苗、腋芽基部30 d左右则直接分化再生芽。经试验筛选出各培养阶段最适宜的培养基为:①愈伤组织诱导培养基,MS+6-BA2.5 mg/L+NAA0.2 mg/L;②芽分化培养基,MS+6-BA3.00 mg/L+NAA0.15 mg/L;③芽增殖培养基,MS+6-BA3.5 mg/L+NAA0.5 mg/L;④生根培养基,1/2 MS+NAA0.5 mg/L。上述培养基均添加浓度2.5%蔗糖,浓度0.8%琼脂,pH值为6.0。

Blade tip, seedling and axillary bud of Aloe saponaria as explants were studied by in vitro culture. Result showed that callus could be generated on the cuts of blade tip at about 25 d. And after 15 d, regeneration buds germinated. Seedling base could directly differentiate into regeneration buds. The optimal culture mediums were selected, which were callus induction medium MS ＋ 6-BA 2.5 mg/L ＋ NAA 0.2 mg/L, bud differentiation medium MS＋6-BA 3.0 mg/L ＋ NAA 0.15 mg/L, bud proliferation medium MS＋6-BA 3.5 mg/L ＋ NAA 0.5 mg/L and root formation medium 1/2 MS ＋ NAA 0.5 mg/L. The culture mediums above contained 2.5 % sucrose and 0.8 % agar with pH value 6.0.