荧光胺示踪氨基葡甘露聚糖的跨细胞膜能力与转运机制

Tracing transmembrane transport of amino-glucomannan

目的用荧光胺标记来示踪氨基葡甘露聚糖(aminoglucomannan,AGM)的跨细胞膜转运,以此证实氨基葡甘露聚糖跨(细胞)膜能力与转运机制。方法用荧光胺标记氨基葡甘露聚糖,将标记所得的荧光衍生物与外周血单个核细胞(peripheral blood mononuclear cells,PBMC)和HepG2细胞在CO2培养箱中孵育,然后用荧光显微镜(紫光作激发光)观察。结果PBMC和HepG2细胞内均出现靛色荧光。结论氨基葡甘露聚糖能跨细胞膜转运进入细胞内。

Objective To label the amino-glucomannan （AGM） with fluram and to explore the properties of the compound of fluram-AGM in transmembrane transport. Methods After AGM was labeled with fluram, the peripheral blood mononuclear cells （PBMC） and HepG2 were respectively cultured with Fluram-AGM, then observed under fluorescence microscope with violet light as exciting light. Results Both PBMC and HepG2 showed intracellular indigotic fluorescence. Conclusion AGM can be transported into cells across cell membrane.