人毛囊细胞来源双层皮肤替代物的构建和组织学特征

Reconstruction and histologic characteristics of bilayer skin substitute inoculated of human hair follicle cells

目的探索人毛囊细胞构建的双层皮肤替代物的组织学特征。方法用毛乳头细胞、真皮鞘细胞分别与外根鞘细胞构建复合壳多糖双层皮肤替代物,分析其体外和移植至大白鼠后的组织学特征。结果毛囊来源细胞构建的皮肤替代物中表皮细胞排列更紧密、角化突出,真皮内可见与表皮相连的上皮细胞柱及球形膨大细胞团形成。结论毛囊细胞是构建皮肤替代物的良好细胞来源。

Objective To study histologic characteristics of bilayer skin substitute reconstructed by cells from human hair follicle and whether the skin appendage can be induced based on the bilayer skin substitute. Methods After composite chitosan bilayer skin substitute was reconstructed with dermal papilla cells and outer root sheath cells or dermal sheath cells and outer root sheath cells, its histologic characteristics was investigated in vitro and after transplanted onto SD albino rats. Results Composite chitosan bilayer skin substitute reconstructed by cells from hair follicle had closely arranged epithelium cells and outstanding cornification; Epithelial cords linked with epidermis could be seen in dermis. However, there was no certain hair follicle-like structure formation either in vitro or in vivo. Conclusion Hair follicle cells are good source for skin substitute reconstruction, but it can not induce skin appendage formation through skin substitute by now .