诺帝对体外内皮细胞三维成型的抑制作用

Restrain effects of Nordy on tubule-like structure formation of endothelial cells in a three-dimensional culture

目的观察自行研制的抗肿瘤制剂诺帝(Nordy)对三维胶原体外培养条件下恶性胶质瘤细胞诱导ECV304细胞血管生成的抑制作用。方法采用不同浓度、不同时相点给药;观测在恶性胶质瘤细胞系SHG-44诱导ECV304细胞生长和小管样结构形成的过程中,ECV304细胞增殖周期、凋亡率以及小管样结构形成数目、大小等指标。比较诺帝对SHG-44细胞诱导ECV304细胞形成小管样结构作用的差异。结果诺帝对SHG-44细胞诱导的体外三维培养ECV304细胞小管形成具有明显的抑制作用,主要表现在细胞增殖周期、凋亡率以及小管样结构形成数目、大小等指标都有不同程度的改变。结论诺帝通过对细胞核增殖的抑制和诱导部分凋亡作用来抑制小管结构的形成,是一种具有抗体外血管生成作用的良好制剂。

Objective To investigate the effects of the new drug Nordy on malignant glioma cells induced tubule-like structure formation of endothelial cells in a three dimensional culture system with collagen coating. Methods The endothelial cells were divided into three groups. Group A were cultured on the DMEM media coated with collagen; group B were cultured in 2：1 （V/V） DMEM to the supernatant of malignant glioma cell culture ; group C, based on the group B, were added with Nordy of different concentrations. The cell cycle and apoptosis of ECV304 cells were determined and the process of tubular formation were observed. Moreover, the parameters such as the forming curves, outer and inner diameter, wall thickness and length of the tubules were measured and compared between groups B and C. Results The cell cycle of endothelial cells were restrained, and some cells were induced to apopt0sis. The tubule-like structure formation was inhibited, and the tubules in group B appeared earlier, larger in diameter and longer in length than group C. Conclusion The tubular formation induced by malignant glioma cells could be restrained by the new drug Nordy.