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慢传输型便秘患者乙状结肠雌激素β受体分布的免疫组化观察 被引量：6

Distribution of ERβ in sigmoid colon specimens of slow transit constipation
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摘要 目的初步观察雌激素β受体(estrogen receptorβ,ERβ)在慢传输型便秘(slow transit constipation,STC)患者乙状结肠中的分布。方法用ERβ免疫组织化学技术观察16例STC患者以及10例正常对照组乙状结肠中ERβ的分布情况,并进行半定量分析。结果ERβ在STC患者以及正常对照组乙状结肠黏膜以及间质细胞中均有不同程度的表达,正常对照组乙状结肠黏膜主要表现为上皮及腺体细胞核及核周细胞质染色,以核周细胞质染色为主。而STC患者主要表现为上皮及腺体细胞核染色为主。与正常对照组相比,STC患者各个区域ERβ均明显减少(P<0·05)。结论STC乙状结肠ERβ数量显著减少,但ERβ减少是原发性的还是继发于某种其他损害仍有待探讨。 Objective To observe the distribution of ERβ in the sigmoid colon specimens from the patients with slow transit constipation （STC）. Methods The expression of ERβ was detected by immunohistochemical staining in formalin-fixed, paraffi rectal cancer at Dukes＇ stage A, n-embedded sigmoid colon tissues from 16 cases of STC, and from 8 cases of 2 cases of rectal villous adenoma （ 10 as control）. The positive ceils were analyzed by computer assistant image analysis system. Results ERβ immunoreactivity was present in normal epithelium and interstitial ceils of the sigmoid colon tissues in the two groups, but nucleus-positive in STC group and cytoplasm-positive in control group. In comparison with the control group, the ERβ immunoreactivity in STC group was much lower （P 〈0. 05）. Conclusion ERβ expression in the sigmoid colon tissues from STC patients decreased significantly.
作者 张林 刘宝华 童卫东
机构地区 第三军医大学大坪医院野战外科研究所普通外科
出处 《第三军医大学学报》 CAS CSCD 北大核心 2007年第12期1246-1248,共3页 Journal of Third Military Medical University
关键词 慢传输型便秘 雌激素Β受体 CAJAL间质细胞 slow transit constipation ERβ interstitial cells of Cajal
分类号 R392.11 [医药卫生—免疫学] R446.8 [医药卫生—诊断学]
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参考文献15

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二级参考文献23

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共引文献24

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引证文献6

二级引证文献38

第三军医大学学报
2007年 第12期

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