脑出血大鼠脑组织MDA、TNF-α含量变化及其依达拉奉干预效应的研究被引量：19

Changes of TNF-alpha and malondialdehyde content in brain tissue and therapeutic effect of edaravone on rats after intracerebral hemorrhage

下载PDF

导出

摘要目的观察3-甲基-1-苯基-2-吡唑啉-5-酮(MCI-186,商品名为依达拉奉)对出血性脑组织的保护作用和可能机制。方法将96只Sprague-Dawley大鼠按随机数字表法分为假手术组、脑出血模型组、依达拉奉治疗组,每组32只。采用自体血脑内注入法制作大鼠脑出血模型,假手术组只进针不注血。依达拉奉治疗组大鼠腹腔注射依达拉奉(3mg/kg),分别于造模前30min及造模后1次/12h。于6、24、72、168h分别用Longa标准测定神经功能缺损;干、湿重法检测脑含水量;酶联免疫吸附法(ELISA)和硫代巴比妥酸法测定血肿周围组织中脑组织TNF-α和丙二醛(malondialde-hyde,MDA)的含量。结果与假手术组相比,脑出血模型组大鼠神经功能缺损评分、脑含水量、TNF-α和MDA含量均显著增高(P<0.05);与脑出血模型组相比,依达拉奉治疗组大鼠神经功能缺损评分、脑含水量、TNF-α和MDA含量显著下降(P<0.05)。结论依达拉奉对出血后脑组织具有保护作用,其机制可能与抑制MDA和TNF-α的活性有关。 Objective To observe the protective effect of edaraven on brain tissue and the therapeutic mechanism in rats following intracerebral hemorrhage（ICH）. Methods In this series, 96 Sprague-Dawley rats were divided into three groups at random（ n = 32 in each group）： sham-operative group, ICH model group and edaraven therapy group. ICH model was induced by infusion of autologous whole blood into the caudate-putamen. Rats in sham-operative group were treated the same as above, but no blood injected. Rats in edaraven therapy group were treated with edaraven （3 mg/kg） by peritoneal injection 30 min before operation and once per 12 hours after operation. These rats were killed at 6, 24, 72, and 168 h after neurologic impairment test with Longa criteria. Brain water content was measured by dry-wet weight method. Malondialdehyde and TNF-alpha in the vicinity of the hematoma were measured by thio-barbituric acid method and ELISA respectively. Results Compared with sham operative group, neurologic impairment score, brain water content, malondialdehyde and TNF-alpha in brain tissues were obviously higher in ICH model group （ P 〈 0.05 ）. Compared with ICH model group, neurologic impairment score, brain water content, malondialdehyde and TNF-alpha in brain tissues were significantly decreased in edaraven therapy group （ P 〈 0.05 ）. Conclusion Edaraven has an evident protective effect on injured brain tissue through inhibiting malondialdehyde and TNF-alpha content in the vicinity of the hematoma.

作者崔洁曹参祥郑静王景周

机构地区第三军医大学大坪医院野战外科研究所神经内科河北省保定市第二医院南院神经内科

出处《第三军医大学学报》 CAS CSCD 北大核心 2007年第11期1032-1035,共4页 Journal of Third Military Medical University

基金重庆市卫生局"十五"重点项目(00-1007)~~

关键词依达拉奉脑出血脑含水量丙二醛肿瘤坏死因子-Α edaraven intracerebral hemorrhage brain water content malondialdehyde tumor necrosis factor-alpha

分类号 R329.25 [医药卫生—人体解剖和组织胚胎学] R743.34 [医药卫生—神经病学与精神病学]

引文网络
相关文献

参考文献10

1Deinsberger W,Vogel J,Kuschinsky W,et al.Experimental intracerebral hemorrhage:description of a double injection model in rats[J].Neurol Res,1996,18(5):475-477.
2Longa E Z,Weinstein P R,Carlson S,et al.Reversible middle cerebral artery occlusion without craniectomy in rats[J].Stroke,1989,20(1):84-91.
3Nakamura T,Keep R F,Hua Y,et al.Oxidative DNA injury after experimental intracerebral hemorrhage[J].Brain Res,2005,1039(1-2):30-36.
4Gasche Y,Copin J C,Sugawara T,et al.Matrix metalloproteinase inhibition prevents oxidative stress-associated blood-brain barrier disruption after transient focal cerebral ischemia[J].J Cereb Blood Flow Metab,2001,21(12):1393-1400.
5Brennan P,O'Neill L A.Effects of oxidants and antioxidants on nuclear factor kappa B activation in three different cell lines:evidence against a universal hypothesis involving oxygen radicals[J].Biochim Biophys Acta,1995,1260(2):167-175.
6张绪清.细胞粘附分子-1表达的基因调控和影响因素[J].国外医学（生理病理科学与临床分册）,1997,17(3):249-251. 被引量：13
7Ding-Zhou L,Marchand-Verrecchia C,Palmier B,et al.Neuroprotective effects of (S)-N-[4-[4-[(3,4-Dihydro-6-hydroxy-2,5,7,8-tetramethyl-2H-1-benzopyran-2-yl)carbonyl]-1-piperazinyl]phenyl]-2-thiophenecarboximid-amide (BN 80933),an inhibitor of neuronal nitric-oxide synthase and an antioxidant,in model of transient focal cerebral ischemia in mice[J].J Pharmacol Exp Ther,2003,306(2):588-594.
8Zhu D Y,Li R,Liu G Q,et al.Tumor necrosis factor alpha enhances the cytotoxicity induced by nitric oxide in cμltured cerebral endothelial cells[J].Life Sci,2000,66(14):1325-1335.
9Cong C,Hoff J T,Keep R F.Acute inflammatory reaction following experimental intracerebral hemorrhage in rat[J].Brain Res,2000,871(1):57-65.
10Edaravone Acute Infarction Study Group.Effect of novel free radical scavenger,edaravone (MCI-186),on acute brain infarction.Randomized,placebo-controlled,double-blind study at multicenters[J].Cerebrovasc Dis,2003,15(3):222-229.