腺病毒介导凋亡素基因对裸鼠种植性人胆管癌的治疗研究

Therapy for xenograft human bile duct cancer in mice mediated by adenovirus containing Apoptin gene

目的探讨腺病毒介导凋亡素基因对裸鼠种植性人胆管癌的治疗。方法建立裸鼠皮下种植性人胆管癌模型,观察肿瘤内注射含凋亡素基因的重组腺病毒后种植瘤的体积变化、毒副作用和组织学变化,同时计算抑瘤率。结果在治疗12d后含凋亡素基因的腺病毒治疗组的种植瘤体积为(92.31±28.31)mm,较空病毒组(288.86±113.13)mm和生理盐水组(344.86±113.87)mm相比具有显著差别(P<0.01)。同时抑瘤率达到72.10%,明显高于空病毒治疗组11.9%(P<0.01)。在治疗期间没有观察到毒副作用。组织学结果也提示凋亡素基因是通过引发胆管癌细胞的凋亡来抑制肿瘤生长的。结论含凋亡素基因的重组腺病毒对胆管癌具有治疗作用。

Objective To study the therapy for xenograft human bile duct cancer in mice mediated by adenovirus containing Apoptin gene. Methods Subcutaneous human bile duct cancer was established in nude mice. Variations of tumor volume and histomorphology, side effects were observed after intratumoral injection of adenovirus containing Apoptin gene. Finally the mice were sacrificed for calculating the ratio of antitumor. Resuits Twelve days after treatment, the mean volume of the xenograft human bile duct cancer in the group of intratumoral injection of adenovirus containing Apoptin gene was （92.31 ± 28.31 ） mm, which was reduced significantly compared with that of adenovirus infection without apoptin gene（288.86 ± 113.13 ）mm and control group（344.86 ± 113.87）mm. The ratio of antitumor was 72.10% , which was significandy higher than that in control group（ 11.9% ）. During the whole experimental course, no side effect was observed. The histological results demonstrated that the reduction of tumor reduced by transfection of Apoptin gene. Conclusion was the result of apoptosis in bile duct cells, which was The adenovirus vectors containing Apoptin gene may constitute a safe tool for the treatment of cholangiocarcinoma.