模拟CO_2气腹对人子宫内膜癌细胞黏附能力的影响

Effect of pseudo CO_2 pneumoperitoneum on cell adhesion capability of cultured endometrium cancer cells

目的探讨二氧化碳(CO2)气腹环境对培养人子宫内膜癌细胞黏附能力的影响。方法体外培养人子宫内膜癌HEC-1-B细胞株,以模拟CO2气腹处理4h,通过MTT法检测细胞生长变化和细胞黏附改变;应用免疫组织化学方法检测细胞中β1-整合素(β1-integrin)以及E-钙黏素(E-cadherin)的表达变化。结果CO2处理4h后,与对照组相比,HEC-1-B细胞生长曲线上移,黏附能力增强,β1-整合素与E-钙黏素的表达均减少。结论CO2气腹有可能通过减弱子宫内膜癌细胞β1-整合素和E-钙黏素的表达导致其黏附能力增加,从而可能对肿瘤细胞的转移产生一定影响。

Objective To investigate the effect of CO2 pneumoperitoneum on the cell adhesion capability of cultured endometrium cancer cells. Methods After treated in a pneumoperitoneum model for 4 h, the changes in cell growth and adhesion of the endometrium cancer were detected by MTF. The expression of β1-integrin and E-cadherin in the cancer cells were compared with the control that were not treated with CO2, by the immunohistochemical method. Results After treated with CO2 for 4 h, the cell growth was enhanced and the cell adhesion capability increased as compared with the control group. At the same time, the expression of β1-integrin and E-cadherin was lower in treated cells than that in untreated ones. Conclusion The adhesion capability of endometrium cancer cells in CO2 pneumoperitoneum could be enhanced by the reduction of the expression of β1-integrin and E-cadherin, and thereby influences the metastasis of cancer.