甜樱桃巨红13-38花药发育时期鉴定及愈伤诱导的初步研究

Preliminary Study on the Identification of Microspore in Different Stages and Callus Introduction of Prunus avium L JuHong13-38

以甜樱桃巨红13-38为试材,对其花药发育时期和不同时期愈伤的诱导进行了研究.结果表明：小孢子母细胞越冬前已经形成,一直持续到次年的2～3月份,3月中旬开始减数分裂,3月末形成四分体,4月初形成单核期小孢子,4月中旬形成成熟花粉;花药单核期时诱导的愈伤状态最好为绿色,较致密,愈伤诱导率是各时期中最高的,为4.828%.

JuHongl3 - 38 （Pnmusavium L）, as the material, was studied for the identification of microspore in different development stages and callus introduction . The results are as follows： the microsporocytes of Pnmus avium L JuHongl3 - 38 formed before the winter and persisted to February and March of the next year. By the mid March, microsporocytes started their meiosis process and resulted in microspore tetrads in the end of March. Uninucleate pollen grains formed in early April and changed into mature pollen in the mid April. The callus in Uninucleate of sweet cherry JuHong 13 - 38 was grassy and compact. The highest callus introduction happened in uninucleate stage and introduction frequency was 4. 828 %.