自动混合呼吸测量仪的开发与验证

Development and validation of automated hybrid respirometer

呼吸测量技术通过测量和解析氧利用速率(OUR)能够反映活性污泥微生物代谢状态、监控废水处理工艺运行状况、获取反应动力学参数和污水组分参数。混合呼吸测量原理即是为满足这些应用而提出的具有高测试精度和频率的OUR测试方法。依据该原理,本研究提出了一种新的具体实现形式。实验证实,所研制的自动混合呼吸测量仪通过测量室的设计解决了流速和流态差异对DO电极测量值的影响,通过温控和磁力搅拌系统实现了系统整体恒温,通过测试软件开发,减小了干扰,提高了测试精度,实现了测量过程可视化。实用结果表明,自动混合呼吸测量仪具有很好的精度和长期稳定性,有较好的应用前景,值得进行深入研究。

The aim of this paper is to realize fully the hybrid respirometric measurement principle with high accuracy and measuring frequency. Based on the knowledge of the difficulty in implementing ＂ultimate＂ hybrid respirometer and the drawbacks of the ＂simpler hybrid respirometer＂, a novel implementation of the hybrid respirometric measurement principle was developed. It was verified that the new hybrid respirometer possessed the advantage of ＂ultimate＂ hybrid respirometer while its DO electrodes suffered no effect of flow rate change. Also the accurate and constant temperature of the whole set-up was solved. For the purpose of enhancing the availability of the respirometer, software with friendly user interface was developed, in which advanced arithmetic and median filter were adopted. Finally the new hybrid respirometer system was evaluated and the accuracy and reliability were satisfied, which showed that the further research is worthwhile.