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BCG-CpG-DNA对重组HBsAg的免疫佐剂作用 被引量：15

Effect of BCG-CpG-DNA as An Immunoadjuvant of Recombinant HBsAg
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摘要 目的研究BCG-CpG-DNA对重组HBsAg的免疫佐剂作用。方法以BCG-CpG-DNA与重组HBsAg混合免疫小鼠,以铝佐剂疫苗为对照,采用放射免疫法检测抗-HBs中和抗体水平,ELISA法分析抗体亚类,酶联免疫斑点试验(ELIS-POT)检测CD8+细胞毒T淋巴细胞(CTL)功能。结果BCG-CpG-DNA能提高抗-HBs中和抗体水平,促进抗原特异性IgG2a的产生,诱导Th1型免疫应答,与铝佐剂协同作用能部分逆转Th2反应。同时能增强抗原特异性CD8+T淋巴细胞的杀伤作用,实验组与对照组比较差异有显著意义。结论BCG-CpG-DNA对HBsAg有较好的免疫佐剂作用,有可能成为一种新型的免疫佐剂。 Objective To study the effect of BCG-CpG-DNA as an immunoadjuvant of recombinant HBsAg. Methods Immunize mice with the mixture of BCG-CpG-DNA and recombinant HBsAg. Determine the anti-HBs neutralizing antibody by RIA, the subclass of antibody by ELISA,the function of CTLs by ELISPOT,and compare with those of HB vaccine containing aluminium hydroxide adjuvant. Results BCG-CpG-DNA increased the anti-HBs neutralizing antibody level, promoted the production of antigen specific IgG2a,induced Thl immune response, and partially reversed Th2 response. The killing activity of antigen specific CD8^＋ T lymphocytes of mice in test group was significantly higher than that in control group. Conclusion BCG-CpG-DNA showed good adjuvanticity to recombinant HBsAg, and might be used as a novel immunoadjuvant.
作者 赵爱华 乔来艳 贾淑珍 寇丽杰 王国治
机构地区 中国药品生物制品检定所菌苗室
出处 《中国生物制品学杂志》 CAS CSCD 2007年第5期356-358,361,共4页 Chinese Journal of Biologicals
关键词 BCG—CpG-DNA 免疫佐剂 HBSAG BCG-CpG-DNA Immunoadjuvant HBsAg
分类号 R979.5 [医药卫生—药品]
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参考文献13

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二级参考文献17

  • 1赵爱华,贾淑珍,乔来艳,寇丽杰,王国治.BCG-CpG-DNA制备、理化特性及免疫刺激活性的初步研究[J].微生物学免疫学进展,2005,33(1):39-43. 被引量：18
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共引文献21

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引证文献15

二级引证文献48

中国生物制品学杂志
2007年 第5期

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