不同方法制备的1型肺炎球菌多糖-蛋白结合疫苗生化及免疫学特性比较

Biochemical and Immunological Characteristics of Pneumococcus Type 1 Conjugate Vaccine Prepared by Cyanogen Bromide Activation and Amine Reduction

目的对两种方法制备1型肺炎球菌荚膜多糖-蛋白结合疫苗的生化及免疫学特性进行比较。方法分别采用溴化氰活化法与胺还原法制备结合物,并对其进行生化及免疫学检测。结果采用溴化氰活化法制备的结合物,较胺还原法具有较高的结合率及高分子结合物含量,免疫小鼠产生的抗体效价较胺还原法明显提高。结论采用溴化氰活化法制备1型肺炎球菌荚膜多糖-蛋白结合疫苗优于胺还原法。

Objective To compare the biochemical and immunological characteristics of pneumococcus type 1 conjugate vaccine prepared by cyanogen bromide activation and amine reduction. Methods Prepare pneumococcus type 1 conjugate vaccine by cyanogen bromide activation and amine reduction respectively and determine their biochemical and immunological characteristics. Results The conjugation rate, high molecular conjugate content of and the antibody titer induced with pneumococcus type 1 conjugate vaccine prepared by cyanogen bromide activation were significantly higher than that by amine reduction. Conclusion Compared with amine reduction, cyanogen bromide activation was more suitable for the preparation of pneumococcus type 1 conjugate vaccine.