HLA-DRB1和DQ基因与胰岛素依赖型糖尿病遗传易感相关性的联合研究

A combinedstudy on the relationship between HLA-DRB1andDQ alleles andthegeneticsusceptibility of IDDM

用ＰＣＲ（聚合酶链反应）及序列特异性寡核苷酸探针杂交技术（ＳＳＯＰＨ）对中国北方人中ＨＬＡ－ＤＲＢ１和ＤＱ基因与ＩＤＤＭ的遗传易感相关性联合进行了研究，以５４例胰岛素依赖型糖尿病患儿为研究对象，４０例正常成年供血员为对照。结果表明：ＤＲＢ１＊０３０１、ＤＱＡ１＊０５０１、０３０１和ＤＱＢ１＊０２０１为ＩＤＤＭ易感性基因，ＤＱＡ１＊０１０３、ＤＱＢ１＊０６０１为ＩＤＤＭ保护性基因。ＤＲＢ１＊０３０１－ＤＱＡ１＊０５０１－ＤＱＢ１＊０２０１为ＩＤＤＭ易感性单倍型。ＤＲ３／ＤＲ４和ＤＲ３／ＤＲ９杂合子在病人中显著增高，表明ＤＲ３和ＤＲ４或ＤＲ３和ＤＲ９单倍型的易感性效果之间产生了协同作用。

PCR and dot blot techniques were usedto classifyHLAalleles soas to studytheaffection to IDDM contributed by HLA-DRB1 and DQ alleles。In our study,54IDDM patients wereselected asresearch subjects and 40normaladult blooddonors as controls.Ourresultsindicated thatthefrequenciesof DRB1* 0301,DQA1*0501,DQA1*0301andDQB1*0201,the susceptible alleles,weresignificantly increased inIDDM patients,and the frequencies of DQA1*0103and DQB1*0601,the protective alleles,significantly increased in controls。DR3/DR4andDR3/DR9heterozygotes increased significantly in IDDM patients.The results indicated thatthe suseptibility offered by DR3 and DR4 or DR3 and DR9 haplotypes had synergetic effect.