糖尿病大鼠骨量及骨形态学变化的研究

A study on the variation about bone mass and morphology in diabetic rats

采用四氧啼晚制成ＳＤ雄性大鼠糖尿病模型，探讨糖尿病及胰岛素治疗后对骨量及骨形态学影响过程。设正常对照组、糖尿病未治组（按病程分ＤＭ４ｗ、ＤＭｅ”、ＤＭ“ｗ）、糖尿病胰岛素治疗组（按治疗时间分Ｔ４Ｗ、Ｔ８Ｗ、Ｔ１２Ｗ）。应用ＳＰＡ、ＳＥＱＣＴ两种方法对其股骨进行骨量测定，表明糖尿病早期即有股骨干皮质骨密度下降，且随病程进展，干骺端松质骨量也显著减少。光镜观察，糖尿病早期即显示股骨颈处皮质变薄、骨小梁细疏。随胰岛素治疗时间延长，骨量有所恢复，骨组织形态也相应得到改善。

s The male Sprague-Dawley rats with alloxan-induced diabetes were used to study the ef- fects of diabetes mellitus and insulin therapy on bone. mass and ultrastructure. The rats were divided in- to three groups : controls , untreated diabetics ( divided into DM4w , DM8w and DM12w according to the course of disease ) and diabetics treated with insulin (divided into T4w , T8w and T12w according to the time of insulin treatment), The results showed that the cortical bone mass in femur measured by SPA was sienificantlv lower early in diabetes , and the trabecular bone mass also decreased in the course of dis- ease. Morphologically th. femoral necks in early diabetes showed decrease of cortical thickness and tra- becular number. With long term insulin therapy , the bone mass increased and the bone ultrastructure improved.