在缺氧条件下培养的海马神经元形态结构及热休克蛋白(HSP)70表达变化及丹参的影响

Changes of Morphology and HSP70 Expression and RSM Effects Following Anoxia in Cultured Hippocampal Neuronal Cells

在体研究曾发现在缺血再灌注模型中，丹参具神经保护作用。本文采用新生大鼠海马神经元培养技术，以细胞形态学及HSP70免疫性细胞表达为指标，首次观察了缺氧条件下培养的海马神经无形态结构及HSP70表达变化及丹参的影响。结果发现：（1）不论缺氧1h或2h，在培养的海马神经细胞中，有的细胞出现细胞体周围光晕消失，胞浆内颗粒变性，细胞膜肥厚、粗糙，轴突变粗、断裂，并且出现HSP70免疫阳性细胞；（2）缺氧1h组的海马神经细胞存活率及HSP70免疫阳性细胞率均显著高于缺氧2h组，（3）丹参组（在缺氧0．5h前给丹参100mg／ml（终浓度）的海马神经细胞存活率及HSP70免疫阳性细胞率均显著高于缺氧2h组。结果提示丹参具有直接的抗缺氧性神经细胞损伤的作用，减轻了缺氧所造成的神经细胞形态学上的改变。

In vivo study we have found that Radix Salviae Miltiorrhicae (RSM) has neuroprotective effect in ischemic-reperfusion models. The purpose of present study was to investigate and confirm whether RSM has direct neuroprotective effect in anoxic cultured natal hippocampal neuronal cells by using morphological changes and heat shock protein 70kDa(HSP70) expression as indicators. Results:(1)after 1 or 2h anoxia, there were neurons with disappearance of halo around cell body, granular degeneration in cytoplasm, nuclear and coarse cell membrane, broken and thickened a-con, and also HSP70 positive cells were demonstrated; (2)the survival neuronal cell rate and HSP70 positive cell rate were significantly higher in 1h-anoxic group as compared with in 2h-anoxic group; (3)in RSM group (0. 5h before anoxia, given RSM with final concentration of 100mg/ml)both neuronal cell survival rate and HSP 70 positive cell rate were significantly higher as compared with 2h-anoxic group. Conclusion RSM has direct neuronal protective effect on anoxic damage in cultured natal hippocampal neurons.