不含甲硫氨酸全肠外营养对大鼠胃癌治疗作用的研究

Studies on Therapeutic efects of L-methionine deprived total parenteral nutrition in gastric cancer bearing rats

实验性胃癌大鼠 2 3只 ,随机分为对照组和治疗组 ,经颈内静脉行上腔静脉插管 ,分别予以含甲硫氨酸和不含甲硫氨酸全肠外营养治疗一周 ,观察其抗肿瘤作用。结果显示 :两组大鼠体重和肿瘤细胞DNA含量均无显著改变 ,治疗组肿瘤直径明显缩小 ,生存期显著延长。细胞周期分析表明 ,两组肿瘤细胞S期比例均显著增高 ,G0 /G1期比例显著下降 ;治疗组G2 M期比例亦明显下降 ,而对照组G2 M期比例增高。组织病理学检查发现 ,治疗组肿瘤组织内坏死灶明显增多 ,存活肿瘤细胞核仁增大。表明不含甲硫氨酸全肠外营养可能通过干扰肿瘤细胞DNA合成和RNA转录 。

In this study 23 gastric cancer bearing rats were devided into control group and experimental group randomly.One group was given TPN containing methionine and another group was given methionine deprived TPN.The anti tumor effects were observed.The results showed that there were no changes in body weight and DNA content of cancer cell in both groups.The mean diameter of tumor was shrink,and the life span was aparently longer in rats receiving no methionine.Cancer cell cycle analysis showed that S phase ratio was significantly increased and G 0/G 1 phase ration was markly decreased in the two groups.The G 2M phase ratio was decreased in no methionine group but was increased in methionine not deprived group.Pathologic examing showed that necrotic foci were increased in the tumor tissue and the nucleolar were enlarged in the no methionine grouop.All the results showed that methionine deprived TPN would inturbance the DNA sythesis and RNA transcription of tumor cells and exsert the anti tumor effects.