摘要
目的探讨转染mtrⅡ基因后的霍奇金病细胞增殖活性的改变及其意义。方法将mtrⅡ转染人霍奇金病细胞系,采用免疫组化法检测转染前后ki67基因、增殖细胞核抗原(PCNA)表达情况。结果未转染mtrⅡ基因的ki67阳性率为21.3%,转染mtrⅡ基因后ki67阳性率为36.8%,两者间有显著性差异(P<0.05);未转染mtrⅡ基因的PCNA阳性率为46.1%,转染mtrⅡ基因后PCNA的阳性率为78.6%,两者间有显著性差异(P<0.05)。结论转染mtrⅡ基因后的霍奇金病细胞增殖活性明显增加,提示mtrⅡ基因可能参与了霍奇金病恶性转化的过程。
Objective To investigate the proliferative activities and significance of Hodgkin＇s disease cells which were transfected with mtr Ⅱ gene. Methods The ki67 and proliferating cell nuclear antigen （PCNA） expression in Hodgkin＇s disease cells transfected with mtr Ⅱ gene were determined with immunohistochemistry. Results The ki67 and PCNA expression in cells transfected with mtr Ⅱ gene was more than that of cells without mtr Ⅱ gene transfected （P〈0.05）. Conclusion The proliferative activities of Hodgkin＇s disease cells transfected with mtr Ⅱ gene increased obviously, which may be related to the progress of Hodgkin＇s disease.
出处
《中国康复理论与实践》
CSCD
2007年第5期430-431,F0003,共3页
Chinese Journal of Rehabilitation Theory and Practice
基金
福建省自然科学资金资助项目(C0410048)
