脂多糖与生物分子的相互作用及动力学分析

Kinetic Analysis of Interaction Between Lipopolysaccharide and Biomolecules

脂多糖(LPS)是构成革兰氏阴性细菌细胞外壁的主要成分,是一种热稳定的毒素。脂多糖与某些生物分子形成复合物,从而引发毒性反应。本研究应用基于表面等离子体共振(SPR)原理的生物传感技术,对脂多糖与几种生物分子之间的相互作用进行了动力学分析。采用氨基偶联法在CM5传感片上固定生物分子,LPS溶液注射于固定的传感片上,测得脂多糖与人血清白蛋白、血红蛋白、壳聚糖以及溶菌酶的亲和常数kA分别为2.36×107、2.03×108、7.58×106、2.82×104L/mol,而与铁蛋白之间没有结合。

Lipopolysaccharide （LPS） is a major component of the outer membrane of all gram-negative bacteria. It is a heat-resistant toxin which can cause toxic shock in animals. LPS interacts with some biomolecules and triggers its toxic reaction. In this study, the interaction between LPS from Salmonella Minnesota and some biomolecules using syrface okasnib resibabce （SPR） biosensor, biomolecules were imobilized on CM5 sensorchip suing amion coupling method and LPS was injected over the immobilized surfaces. The affinity constants kA of LPS with serum albumin, hemoglobin, chitosan and lysozyme were 2.36 × 10^7, 2.03 ×10^8, 7.58 × 10^6, 2.82 ×10^4 L/mol, resoectivelv. But LPS could not interact with ferritin.