蛋白质相互作用的研究方法

Development of Analytical Techniques for Protein-Protein Interaction

生物体的生理功能主要由细胞中的蛋白质控制和调节。其中,多数蛋白质是通过与配体结合或是作为蛋白质复合物中的一部分参与细胞的代谢过程。因此,研究蛋白质间的相互作用是理解生命活动的基础。本文对现有蛋白质相互作用的研究方法和技术进行了评述。

The physiological functions of organisms, including humans, are mainly controlled and modulated by proteins in cells. Most of proteins firstly combine with ligand or form protein complex with other proteins, and then participate in the process of cell metabolism. Hence, protein-protein interactions are fundamental to understand biological processes. This paper reviewed the published techniques and methods for protein-protein interaction.