高葡萄糖促进人内皮细胞NOX4表达

High Concentration of Glucose Induced NADPH Oxidase 4 Expression of HUVECs

目的观察高葡萄糖刺激HUVECs时NOX4表达水平的变化。方法倒置显微镜下观察人脐静脉内皮细胞形态;用免疫组织化学法检测人脐静脉内皮细胞Ⅷ因子相关抗原的表达;实验设对照组(给予含生理浓度葡萄糖的基础培养液培养)、高葡萄糖组(30 mmol/L葡萄糖处理16 h)和DPI干预组(30 mmol/L葡萄糖加10μmol/L的NADPH氧化酶抑制剂DPI处理16 h),用RT-PCR检测NOX4 mRNA水平。结果HUVECs中Ⅷ因子相关抗原表达为阳性;高葡萄糖组的HUVECs中NOX4 mRNA表达上调与对照组相比差异有显著性;而DPI干预组的HUVECs中NOX4 mRNA表达与对照组相比差异无显著性。结论高葡萄糖能促进HUVECs中NOX4 mRNA表达,DPI能抑制HUVECs中NOX4 mRNA表达。

Objective To investigate the change of NOX4 mRNA expression in HUVECs stimulated by high concentration of glucose. Methods Morphology of HUVECs was observed by inverted microscope, expression of factor Ⅷ related antigen in HUVECs was investigated by immunohistochemistry. HUVECs were divided into three groups： control group（ treated with physiological concentration of glucose for 16 hours）, high glucose group（treated with 30 mmol/L glucose for 16 hours） and DPI intervention group（ treated with 30 mmol/L glucose plus 10μmol/1 DPI for 16 hours）, mRNA expression of NOX4 was detected by RT- PCR. Results The results showed that there was expression of factor Ⅷ related antigen in HUVECs. mRNA expression of NOX4 was increased in HUVECs stimulation with high glucose （ P 〈 0.05, vs control group ）. After treated with DPI, mRNA expression of NOX4 in HUVECs decreased（ P 〉 0.05, vs control group ）. Conclusions The mRNA expression of NOX4 in HUVECs increased significantly stimulated by high glucose, while DPI could decrease NOX4 mRNA expression in HUVECs