硫化氢对H_2O_2损伤PC12细胞的保护作用

Protective Effect of Hydrogen Sulfide on H_2O_2-Induced Damage in PC12 Cells

目的探讨硫化氢对氧化应激损伤PC12细胞的保护作用。方法以H2O2损伤PC12细胞为氧化应激损伤的模型,甲氮甲唑蓝法检测细胞增殖状况;Hoechst荧光染色观察细胞形态和核形态;碘化丙啶染色、流式细胞术检测细胞凋亡。结果硫化氢可明显减少H2O2诱导的PC12细胞核呈浓染致密的固缩形态或颗粒状荧光的细胞数;200和400μmol/L硫化氢均可降低200和400μmol/L H2O2作用24 h后对PC12细胞生长的抑制率,明显抑制200和400μmol/L H2O2作用24 h后对PC12细胞凋亡的诱导作用。结论硫化氢对氧化应激损伤PC12细胞具有保护作用。

Objective To study the protective effect of hydrogen sulfide （H2S） on PC12 cells damage induced by oxidative stress. Methods Using H2O2 - induced PC12 cells damage as the model of neuron damage induced by oxidative stress,the cell shape and nuclear morphology were observed by nuclear staining with hoechst 33258, the proliferation of PC12 cells was observed by 3 - （4,5 - dimethylthiazol- 2 - yl） - 2, 5 - diphenyltetrazolium bro- mide（ MTT）assay, the apoptosis of PC12 cells was detected by propidium iodide stain flow cytometry （FCM）. Results Exposure to H2O2, the apoptosis of PC12 cells preconditioned by H2S as measured by hoechst stain was significantly decreased, compared with no - preconditioned cells. In the present of H2S （200 and 400 μmol/L）, the inhibitory rates of PC12 cells induced by H2O2 from 200 to 400 μmol/L and the apoptosis of PC12 cell induced by 200 and 400 μmol/L H2O2 for 24 h were decreased. Conclusions H2S has the protective effect on PC 12 cells damage induced by oxidative stress.