甲醛与苯系物对人胚肺细胞联合毒性的作用

Combination Toxicity of Benzene,Toluene,Xylene and Formaldehyde on Human Embryo Lung Cell

目的探讨甲醛及苯系物对人胚肺细胞的联合毒性及联合毒作用类型。方法以不同浓度毒物及其混合物对人胚肺二倍体细胞染毒,采用MTT法测各浓度组人胚肺细胞存活率,根据线性回归法求出各毒物及其混合物半数致死浓度(OLC50),以Finney法求出各毒物及其混合物预计半数致死浓度(PLC50),根据预计半数致死浓度与实际半数致死浓度的比值Q判断各毒物联合毒作用类型。结果4种化合物对人胚肺细胞的LC50分别为甲醛0.267×10-3mmol/mL、苯0.086 mmol/mL、甲苯0.081 mmol/mL、二甲苯0.090 mmol/mL。甲醛与苯系物混合染毒Q值在0.5到2.6之间,苯与甲苯、二甲苯混合染毒Q值<0.5,甲苯、二甲苯混合染毒Q值>2.6。结论4种化合物毒性大小顺序是甲醛>苯>甲苯>二甲苯,与其体外毒性大小顺序基本一致;甲醛与苯系物之间联合毒性呈相加作用,苯可以拮抗甲苯、二甲苯的毒性作用,甲苯、二甲苯之间为协同作用。

Objective To investigate the joint effects of benzene, toluene, xylene and formaldehyde. Methods Human fetal lung cells were exposed to different concentrations of toxicants and their mixtures. MTT assay was used to measure the survival rate. Median lethal concentration （ OLC50 ） of each toxicants and their mixtures were found according to the linear regression method. Predicted lethal concentration （PLC50） of the toxic mixtures were derived by finney law. The joint effects of these toxic mixtures were judged depending on the Q value （PLC50/ OLC50）. Results Four compounds on human embryonic lung cells LC50 were respectively： formaldehyde： 0.267 × 10^-3 mmol/mL, benzene： 0.086 mmol/mL, toluene： 0.081 mmol/mL, xylene： 0.090 mmol/mL. Q value exposed to formaldehyde and benzene toluene xylene were between 0.5 to 2.6, Q value exposed to benzene and toluene xylene were less than 0.5 and Q value exposed to xylene and toluene was beyond 2.6. Conclusions The lung cell toxicity sequence of these four kind of compounds is formaldehyde 〉 the benzene 〉 the toluene 〉 the xylene, they are basically consistent with the order of their vitro toxicity. The joint effects of formaldehyde and benzene toluene xylene are additional action. The joint effects of formaldehyde and benzene toluene or xylene are antagonistic action. The joint effects of toluene and xylene are synergistic action.