聚己内酯电纺纤维的制备及生物相容性研究

Study on preparation and biocompatibility of polycaprolactone nanofibers by electrospinning

目的:评价聚己内酯电纺纤维的生物相容性,对其在组织工程支架方面的应用前景进行探讨。方法:采用静电纺丝法制备聚己内酯纳米纤维,场发射扫描电镜观察其表面形貌及内部结构,通过溶血实验、粘膜刺激实验和细胞毒性实验(MTT法)初步评价其生物相容性。结果:场发射扫描显微镜观察显示所获得纤维呈无纺多孔网状结构,直径范围为153～612nm,纤维形态光滑均一。溶血实验显示材料无溶血现象,不影响凝血功能;MTT试验显示L929细胞在材料浸提液种中生长良好,细胞毒性为0级;粘膜刺激实验未见异常组织学反应。结论:聚己内酯电纺纤维具有良好的生物相容性,对其在组织工程支架材料领域的应用有进一步研究的价值。

Objective To evaluate the biocompatibility of poly （E-caprolactone） （PCL） elecrospun nanofibers and discuss its potential in the applications of tissue engineering scaffold material. Methods Polycaprolactone（PCL） nanofibers were prepared by electrospinning technology. The morphology of nanofibers were observed and using Scanning Electron Microscopy. The hemolysis test, cytotoxicity test, oral mucous membrane irritation test, and acute systemtic toxicity test were performed respectively. Results The nanofibers have a porous and interwoven structure. The diameter distribution is 153nm-612nm. The fibers are smooth and uniform. The scaffold had no hemolytic activities. The level of cytotoxicity of this material was grade O, and no local response including histopathological findings was observed in the oral mucosa membrane irritation test. The acute systemtic toxicity test results showed that no histopathological findings was observed in the vital organs as heart, kidney and liver of tested animals. Conclusion The prepared nanofibers have good biocompatibility and have potential in the applications for tissue engineering fields.