妥泰、丙戊酸钠对成年及幼年大鼠血清瘦素和胰岛素水平的影响

Effects of topiramate and valproate acid on serum insulin and leptin levels in young and adult rats

目的研究并比较妥泰、丙戊酸钠对成、幼年大鼠血清瘦素、胰岛素水平的影响。方法健康的幼年雌性SD大鼠（生后21d）及成年雌性SD大鼠（生后2月）随机分为幼年对照组、幼年妥泰组（幼TPM）、幼年丙戊酸钠组（幼VPA）、成年对照组、成年妥泰组（成TPM）、成年丙戊酸钠组（成VPA）6组，每组10只，对照组每日直接灌胃生理盐水1～2mL，TPM组每日直接灌胃给药TPM50mg／kg，VPA组每日直接灌胃给药VPA200mg／kg，干预5周后处死，取血清标本，利用放射免疫分析法检测血清瘦素及胰岛素水平。结果成TPM组、幼TPM组空腹瘦素、胰岛素水平均分别低于对照组，差异有显著性（P〈0．05），其中以成年组瘦素、胰岛素水平下降显著（P〈0．05）。相反，成年VPA组、幼年VPA组空腹瘦素、胰岛素水平均分别高于对照组，差异亦有显著性（P〈0．05），其中以幼年组瘦素、胰岛素水平增高显著（P〈0．05）。结论TPM可显著降低成、幼年大鼠的血清瘦素及胰岛素水平，以成年组下降显著；VPA可显著增高成、幼年大鼠的血清瘦素及胰岛素水平，以幼年组增高显著。

Objective To study the effects of topiramate （TPM） and valproate acid （VPA） on serum insulin and lepfin levels in young and adult rats. Methods Thirty healthy female young rats （21 days old） and thirty healthy female adult rats （2 months old） were randomly administered with TPM （50 mg/kg daily）, VPA （200 mg/kg daily） or normal saline （control group ） by intragastric administration for 5 weeks. After 5 weeks, serum leptin and insulin levels were detected by radioimmunoassay （RIA）. Results Serum leptin and insulin levels in both the young and adult TPM groups were remarkably lower than those of the corresponding control group （ P 〈 0.05 ）. The adult TPM group had significantly lower serum leptin and insulin levels than the young TPM group （ P 〈 0.05 ）. In contrast, serum leptin and insulin levels in both the young and adult VPA groups were remarkably higher than those of the corresponding control group （ P 〈 0.05 ）. The young TPM group had significantly higher serum leptin and insulin levels than the adult TPM group （ P 〈 0.05 ）. Conclusions TPM decreases serum leptin and insulin levels in young and adult rats, especially in adult rats. VPA increases serum levels of both in young and adult rats, especially in young rats.