肝细胞生长因子在正常大鼠腰段脊髓和背根神经节的表达

Expression of Hepatocyte Growth Factor in the Spinal Cord Lumbar Segments and Dorsal Root Ganglion in Rats

目的:观察肝细胞生长因子(hepatocyte growth factor,HGF)在大鼠脊髓和背根神经节(dorsal root ganglion,DRG)的表达。方法:取健康成年6只SD大鼠运用免疫组织化学染色技术检测HGF在腰段脊髓、背根神经节内的表达和分布。结果:在L4-6段脊髓,HGF免疫阳性产物可见于各板层神经元,尤以脊髓前角运动神经元明显;在DRG中,HGF免疫阳性物质可见于以大、中型为主的神经元的胞浆及突起中。结论:脊髓和背根神经节内的HGF通过与受体c-Met结合可能在神经再生及突触可塑性方面起一定作用。

Objective： To observe the expression of hepatocyte growth factor （HGF） in the spinal cord and dorsal root ganglion （DRG） of rats. Methods：6 adult healthy SD rats were obtained, and the expression and distribution of HGF in the lumbar spinal cord and DRG were detected by immunohistochemical method. Results：Immuno-posifive product of HGF was expressed in degree in each lamina of spinal cord lumbar segments LA-6, especially in the motoneurons of anterior horn; while the expression of immuno-positive product of HGF was detected predominandy in endochylema and ecphyma of large and middle--size DRG neurone. Conclusion：HGF in the spinal cord and DRG, by binding to the cMet receptor, may play a certain role in nerve regeneration and synapdc plasticity.