灵芝多糖液体发酵条件的研究

Study on Fermentation Conditions of Ganoderma Lucidum

液体发酵灵芝是目前灵芝多糖开发的有效途径。以灵芝的生物量和胞外多糖为指标,对影响灵芝发酵的条件进行了研究。单因素实验表明灵芝发酵的最佳碳源、(?)源和生长因子分别是葡萄糖、酵母膏和维生素B1,最适温度、起始pH值和摇床转速分别是28℃、5．5和160 rpm。最佳培养方式是接种后静置4 h再振荡培养,其生物量和胞外多糖的产量最高,分别为7．743g/L和0．907g/L。

The fermentation of ganoderma is the better way to get the ganoderma polysaccharide, The fermentation conditions of ganoderma were studied by assaying the ganoderma biomass and polysaccharide yield. Then, the suitable fermentation conditions were determined as follows： carbon： glucose, nitrogen： yeast exact, growth factor：VB1, temperature 28 ℃, initial pH 5.5, shaking speed 160 rpm. Under the condition of still culture 4 h before oscillation, the biomass and polysaccharide yield reached the highest amount 7.743 g/L and 0.907 g/L respectively.