摘要
目的研究大孔吸附树脂分离纯化山楂叶中总黄酮和金丝桃苷的工艺条件。方法采用高效液相色谱法测定山楂叶提取物中金丝桃苷的含量;紫外-可见分光光度法测定山楂叶提取物中总黄酮的含量。结果D101型树脂对山楂叶中总黄酮和金丝桃苷均有很好的吸附和洗脱性能,洗脱率迭91．20%和98．25%。结论D101型大孔吸附树脂可用作山楂叶提取物总黄酮和金丝桃苷的精制。
OBJECTIVE To optimize the macroporous resin for separating hyperin from Folium Crataegi. METHODS HPLC was employed to determine the content of Folium Crataegi to hyperin. RESULTS absorption content of type HPD100 is the highest one ,but the type D101 was the best for disabsorping hyperin from macroporous resin , the disabsorption was 98.25%. CONCLUSION D101 macroporous resin is effective to separate the hyperin.
出处
《海峡药学》
2007年第5期53-55,共3页
Strait Pharmaceutical Journal
关键词
山楂叶
金丝桃苷
总黄酮
大孔吸附树脂
Folium crataegi
Hyperin
Total flavonoids
Macroporous adsorption resin
