杜仲及其保健品中生物活性成分的毛细管电泳/电化学检测

Determination of Bioactive Ingredients in Eucommia Ulmoides Oliv.and Related Healthy Food by Capillary Electrophoresis with Electrochemical Detection

采用毛细管电泳／电化学检测法（CE／ED）同时分离测定了杜仲叶、杜仲皮及市售杜仲保健品中芦丁、抗坏血酸、金丝桃甙、绿原酸、槲皮素等多种生物活性成分的含量，考察了运行缓冲液酸度和浓度、分离电压、氧化电位和进样时间等实验参数对分离、检测的影响。在最优化条件下，以300μm碳圆盘电极为检测电极，检测电位为＋950 mV（vs .SCE），50mmol／L硼砂的运行缓冲液（pH9．0）中，上述各组分在20min内可基本实现基线分离。各组分浓度与峰电流在3个数量级范围内呈良好线性，检出限（S／N=3）在3．3×10^-5-9．6×10^-5g／L范围。该方法已成功地应用于杜仲及其保健品中生物活性成分的测定，结果令人满意。

A method was developed for the determination of five bioactive ingredients, including rutin, ascorbic acid, hyperoside, chlorogenic acid, and quercetin in Eucommia ulmoides Oliv. and its related healthy food by capillary electrophoresis with electrochemical detection（CE/ED）. The effects of several factors such as the acidity and concentration of running buffer, the separation voltage, the applied potential and the injection time on CE/ED were investigated. Under the optimum conditions, the analytes could be well separated within 20 min in a 75 cm length capillary at a separation voltage of 16 kV in a 50 mmol/L borate buffer solution （ pH 9.0 ）. Good linear relationship was established between peak current and concentration of analytes over three orders of magnitude with detection limits （S/N = 3 ） ranging from 3.3 ×10 ^-5 to 9.6 × 10^ -5 g/L for all analytes. The proposed method has been successfully applied to the determination of the active ingredients in Eucommia ulmoides Oliv. and related products with satisfactory results.