离子色谱-积分脉冲安培检测法测定大蒜多糖的单糖组成

Determination of Monosaccharides in Garlic Polysaccharide by Ion Chromatography-Integrated Pulsed Amperometric Detection

采用氨基酸分离柱（Amino PAC PA10，2×250mm），用10．0mmol／LNaOH溶液作为流动相，以Au为工作电极，Ag／AgCl为参比电极的脉冲积分安培离子色谱法，分离检测了大蒜多糖水解产生的单糖成分及相对含量。实验结果表明，大蒜多糖中含有半乳糖、葡萄糖、甘露醇和果糖，检出限在0．3～6μg／L范围，样品加标回收率为95％～112％。该法具有灵敏度高、精密度好、分离效果好和样品不需要衍生处理的优点，适用于各种植物多糖的单糖组成分析。

Monosaccharides produced via hydrolysis of Garlic polysaccharide were separated on an Amino PAC PA10（2 ×250 mm） column using 10 mmoL/L NaOH as the mobile phase and determined with integrated pulsed amperometric detection （IPAD） using gold as the working electrode and Ag/AgCl as the reference electrode. The results showed that the garlic polysaccharide contained galactose, glucose, mannose and fructose. Their detection limits ranged from 0.3 μg,/L to 6 μg,/L and recoveries ranged from 95% to 112% . The developed method is sensitive and reliable.