叶下珠复方对人肝癌HePG_2细胞体外增殖抑制及诱导凋亡作用

In-vitro Inhibitory Effect of Phyllanthus Urinaria L Compound on Proliferation of Human liver Cancer Cell HePG_2 and Its Apoptosis Induction

目的观察叶下珠复方对人肝癌HePG2细胞体外增殖的抑制作用并探讨其作用机制。方法采用四甲基偶氮唑蓝比色法(MTT)和生长曲线测定法,比较不同浓度的叶下珠复方在不同作用时间对体外培养的HePG2细胞增殖的影响;同时应用流式细胞术、荧光显微镜和电镜对HePG2细胞的凋亡率及形态学进行观察。结果叶下珠复方对HePG2细胞增殖有抑制作用,在一定范围内,叶下珠复方的浓度越大、作用时间越长,对HePG2的抑制作用越强,叶下珠复方浓度为500μg/mL作用72h时,其抑制率达93.58%,IC50为240μg/mL。不同浓度的叶下珠复方均具有一定程度诱导HePG2细胞凋亡的作用。结论叶下珠复方在体外能一定程度抑制肝癌细胞增殖,其机制可能与诱导肝癌HePG2细胞凋亡有关。

Objective To investgate the inhibitory effect of Phyllanthus Urinaria L compound on proliferation of hepatoma cell HePG2 in vitro and to explore its mechanism. Methods The influence of different concentrations of Phyllanthus Urinaria L compound at different time on HePG2 proliferation was compared by MTF colorimetric assay and cell growth curve assay. The cell apoptotic rate and morphological changes of HePG2 were observed by flow cytometry, fluorescence microscope and electron microscope. Results Phyllanthus Urinaria L compound can inhibit the proliferation of human hepatoma cell line HePG2. Within a certain limit of concentrations, the higher the concentration and the longer the acting time, the stronger the inhibition. Co - cultured with 500 μg/mL Phyllanthus Urinaria L compound for 72 h, the inhibitory rate reached 93.58 % and IC50 was 240 μg/mL. Phyllanthus Urinaria L compound at different concentrations had an certain effect on inducing cell apoptosis. Conclusion Phyllanthus Urinaria L compound can inhibit the proliferation of hepatoma cell, and its mechanism may be related with the induction of hepatoma cell HePG2 apoptosis.