香料烟粉虱传双生病毒的分子检测

Molecular detection of whiteflies-transmitted geminiviruses infecting uriental tobacco

发生在保山香料烟生产区粉虱传双生病毒病,已严重影响到了香料烟的品质和产量。本研究利用PCR技术快速检测香料烟植株及田间其它植物如胜红蓟、赛葵及菜豆等、以及病毒介体的带毒情况。对部分样品的PCR检测及序列分析表明,感染香料烟双生病毒有中国番茄黄曲叶病毒、番茄黄曲叶病毒、云南烟草曲茎病毒、烟草曲茎病毒以及云南烟草曲叶病毒等,且一些样品存在复合侵染现象。本研究为掌握病害发生流行的基本规律,监控病害在香料烟上的发生流行提供了依据。

The occurrence of Whiteflies-Transmitted Geminiviruses（WTGs） disease in Bacshan＇ s oriental tobacco planting areas has a severe effect on the quality and yield of oriental tobacco. This study detected rapidly WTGs infecting oriental tobacco, and weeds such as Ageratum, malvastram and bean surrounding the oriental tobacco field,and vector-transmitted WTGs by PCR. PCR detection and sequence analysis of some samples revealed WTGs infecting oriental tobacco consist of Tomato yellow leaf cud China vires ,Tomato yellow leaf curl virus ,Tobacco curly shoot Yunnan virus,Tobacco curly shoot virus and Tobacco leaf curt Yuonan vires. Some samples were infected complexly by these viruses. This study provides the research foundation for holding the basic mechanism of the occurrence and prevalence of WTGs and to monitor the disease.