不产子实体蛹虫草单孢子分离株的获得及RAPD分析

Single spore strains without producing fruit body isolated from Cordyceps militeris and their RAPD analysis

对优势蛹虫草原始菌株WWM04进行单孢子分离,共获得9个单孢子分离株,采用米饭培养基培养确证并且获得6个完全不产子实体的单孢子分离株。利用随机扩增多态性DNA(RAPD)分子标记方法对菌落差异的4个菌落特征典型的不产子实体单孢子分离株(SSP2、SSP7、SSP19和SSP21)、原始菌株以及由原始菌株12次转接后退化的菌株进行遗传多样性分析。结果表明,引物S37、S61对6个菌株的扩增谱带具有非常明显的特异性,该结果为蛹虫草退化分子标记奠定基础,并且在一定程度上也印证了蛹虫草异核现象和准性生殖理论。

6 single spore strains without producing fruit body were isolated from the original Cordyceps militeris, WWM04 with producing fruit body. The genetic diversity of SSP2, SSPT, SSP19 and SSP21 strains without producing fruit body, the original strain and the strain after 12 transferred from the original strain was analyzed by the RAPD method. The results show that the primers S37 and S61 are of apparently specific to the amplified bands of six strains,which establishes the basic for the degenerate molacular marker of Cordyceps millteris and confirms heterokaryosis and parasexuality of Cordyceps militeris, to some extent.