酸、碱溶液对脱脂蚕蛹的解剖结构影响

Influence of acid and alkaline solutions on structure of defatted silkworm chrysalis

将脱脂蚕蛹分别置于清水、1%盐酸溶液和1%氢氧化钠溶液中,100℃浸泡处理20 min,采用电子光学显微分析技术观察脱脂蚕蛹的解剖结构。结果表明:①清水浸泡,蛋白质、甲壳素膨胀;②盐酸溶液浸泡,在蛋白质、甲壳素膨胀的同时,有蛋白的部分分解;③氢氧化钠溶液浸泡,蛋白质、甲壳素膨胀,蛋白质溶解,并伴有部分蛋白的分解。碱对蛋白层与甲壳层的层间有劈开作用。碱液能实现蛋白层与甲壳层的剥离。

In this paper,defatted silkworm chrysalis was treated about 20 min in water,hydrochloric acid solution（1%）and sodium hydroxide solution （1%） respectively and the structure of defatted silkworm chrysalis was observed by electron optical microscope analysis after treatment. The results showed that： ①The protein and chitin expanded after the treatment in water;②Soaked in hydrochloric acid solu-tion,the protein and chitin expanded while a part of protein decomposed;③Soaked in sodium hydroxide solution（1%）, chitin expanded while protein dissolved and decomposed meanwhile; alkalis played an im-portant role in cleaving the interlayer between protein and chitin and could peel off the interlayer of pro-tein and chitin.