摘要
以石杉碱甲为对照品,采用紫外分光光度法在313 nm处测定蛇足石杉中总生物碱含量。结果表明,测定在4.36-21.80μg/mL范围内线性关系良好,回归方程:A=0.026 17C-0.023 14,R=0.999 18(n=7),平均加标回收率为103.2%,相对标准偏差(RSD)为2.13%。实验结果表明,用紫外分光光度法测定蛇足石杉中总生物碱含量具有稳定性好、方法简便的优点。
The determination was conducted by ultraviolet spectrophotometry photometer under 313 nm ultraviolet radiation,while the Huperzine A was selected as contrast substance.The results show：the linearity relation between absorbency and content is good when the content is 4.36-21.80μg/mL,and the regression equation is A=0.026 17C-0.02314,R=0.99918（n=7）,average complementary post recovery rate 103.2%,RSD=2.13%.Ultraviolet spectrophotometry is simple and quick method to determine the content of the total alkaloids in Huperzia serrata with good stability.
出处
《应用化工》
CAS
CSCD
2007年第5期501-502,共2页
Applied Chemical Industry
基金
四川省教育厅青年基金项目(2006B052)
