摘要
目的建立司帕沙星分散片中司帕沙星的高效液相色谱测定方法。方法ODS-C18柱(4.6mm×200mm,5μm);0.05mol.L-1十二烷基硫酸钠溶液(磷酸调pH至3.0)-乙腈(60∶40)为流动相;流速1.0mL.min-1;检测波长275nm。结果司帕沙星浓度在30～70μg.mL-1范围内,与峰面积呈良好的线性关系,r=0.9998,平均回收率为98.8%(n=9)。结论该方法可用于司帕沙星分散片的质量控制。
OBJECTIVE To establish an HPLC method for determination of sparfloxacin in Sparfloxaein Dispersible Tablet. METHODS An HPLC method was developed. Chromatorgraphic assay was performed on a column of ODS-C18（4.6mm×200mm,5μm）, using the mobil phase of 0. 05mol · L^-1 sodium dodecyl sulfate（adjust to pH3.0 with phosphoric acid）- acetonitrile（60 ： 40）at the wavelength of 275nm with the flow rate of 1mL · min^-1. RESULTS The calibration curve of Sparfloxacin was linear in the concentration range of 30-70μg·mL^-1 The average recovery was 98.8% （n = 9） CONCLUSION The established method can be used for the quality control of Sparfloxacin Dispersible Tablet.
出处
《齐鲁药事》
2007年第5期285-286,共2页
qilu pharmaceutical affairs
