乙酰唑胺对缺氧耐受小鼠血清T-chE和T-AOC的影响

Effects of acetazolamide on the ability of anti-anoxia and the vitality of T-ChE and T-AOC in serum in mice

目的:研究乙酰唑胺对小鼠缺氧耐受的影响和机制。方法:小鼠随机分四组:生理盐水对照组、心得安对照组、乙酰唑胺高剂量组和乙酰唑胺低剂量组。高剂量组予以乙酰唑胺1g/kg、低剂量组予以乙酰唑胺0.2g/kg灌胃,阳性对照组给予心得安0.16g/kg灌胃,正常对照组用20g/kg生理盐水灌胃,各组小鼠均每天灌胃一次,连续5天并于末次给药1小时后测定小鼠在室温空调25℃条件下的常压缺氧耐受能力;同时在小鼠死亡时尽快断头取血,以3000r/min,离心5分钟,按试剂盒说明书测定血清胆碱酯酶(T-ChE)活性和总抗氧化能力(T-AOC)。结果:乙酰唑胺低剂量组和高剂量组小鼠耗氧率均较生理盐水组显著降低(均P<0.01),与心得安组比较差异有显著性(分别P<0.01和P<0.05);乙酰唑胺高剂量组T-ChE活性和T-AOC均明显比生理盐水组高(均P<0.01),比心得安组显著增高(分别P<0.01和P<0.05)。结论:乙酰唑胺高、低剂量均能提高小鼠抗常压的缺氧耐受能力,与提高T-AOC和T-ChE活性有关。

Objective： To study the effects and mechanism of acetazolamide on the ability of anti-anoxia in mice.Methods：Mice were divided into four groups at random.Acetazolamide high dose group was intragastric administration with acetazolamide 0.1 g/kg/d but the low dose 0.02 g/kg/d, Propranolol group was intragastric administration with Propranolol 0.16 g/kg/d, and saline group was intragastric administration with saline 20 g/kg/d, all the mice were treatmed for 5 days. At last time, the mice were put in a jar to test the ability of anti-anoxia ＇after intragastric administration for 1 hour,and the serum activity of T-ChE and T-AOC was measured respectively. Results：Both of acetazolamide high dose and low dose groups were improved the ability of ant-anoxia very significantly （both P〈0.01）, and the T-ChE and T-AOC activities of the serum were improved significantly （all P〈0.01）. Conclusion：The acetazolamide can improve the ability of anti-anoxia by improving the T-AOC and T-ChE activities.