肾淋巴循环障碍对大鼠肾功能和组织结构的影响

Role of renal lymph circulation disorder on renal function and structure in rats

目的:观察肾淋巴循环障碍对大鼠肾脏功能及组织结构的影响。方法:雄性Wister大鼠48只,随机分为模型组和假手术对照组各24只。每组分别在术后1、2、4、8周各处死6只。测定24 h尿蛋白和血肌酐。PAS和Masson染色观察肾组织病理改变,并行半定量分析。透射电镜观察肾脏超微结构改变。结果:模型组大鼠尿蛋白显著增加,且随时间推移肾功能逐渐减退,并出现明显的组织病理改变,尤其小管损伤指数及小管间质纤维化指数明显高于对照组(分别为P<0.01和P<0.05)。电镜结果显示,肾小球内皮细胞中线粒体多出现低密度或空化,系膜插入,局部足突细胞融合。结论:肾淋巴循环障碍可致大鼠肾脏功能及小管间质损害,并随时间延长而加重,提示肾淋巴循环障碍为引起慢性肾损害的原因之一。

Objective： To investigate the influence of renal lymph circulation disorder on renal function and structure in rats. Methods： Forty-eight male Wister rats were randomly divided into two groups, twenty-four in each： the sham operation group （ the control group） and the renal lymph ligation group （the model group）. Six rats in each group were sacrificed at 1, 2, 4 and 8 weeks after operation. Renal tissues were determined by PAS and Masson staining, and renal lesions were determined by semiquantitative scores. The ultra-structure of the renal tissues was determined by an electron microscope. Results： Renal disfunetion and tubulointerstitial fibrosis were found in the model group. Tubular damage and tubulointerstitial fibrosis were remarkably aggravated in the model group compared with the control group （P〈0.01 and P 〈 0.05, respectively）. Mitochondrion cavitation, mesenterium insertion and foot cell fusion were found in the model group by an electron microscope. Conclusion： These results indicate that a disorder of renal lymph circulation leads to chronic renal failure and structural lesions, especially for tubulointerstitial fibrosis.