摘要
棉花纤维在延伸的开始以后很快伸长,最后导致大量纤维素的存款。为了在快延伸和第二等的细胞壁合成揭示棉花纤维房间的特征,上演,我们比较了 respectivetranscriptomes 和代谢物侧面。由 cDNA 数组的 transcriptomes 的比较分析识别了是在纤维开发期间调整的差别的 633 基因。用变量把纤维样品划分了成四的表示基因的 Principalcomponent 分析(PCA ) 组织,它发育阶段是诊断的。如果,类似的组织结果也被发现我们是的 usenon 极或极的代谢物为开发纤维的 PCA 的变量。植物生长素发信号,松开墙并且脂类代谢在纤维延伸期间是高度活跃的,而纤维素生合成是占优势的,许多另外的新陈代谢的小径是在第二等的细胞壁合成调整的 down,上演。抄本和代谢物侧面和酶活动在向纤维素合成表明棉花纤维开发的一个专门化过程是一致的。这些数据证明在某个阶段的那个棉花纤维房间有它棉花纤维房间的自己的唯一的特征,和发育阶段能被他们的抄本和代谢物侧面区分。在第二等的细胞壁合成阶段期间,新陈代谢的小径被流进纤维素合成。
Cotton fibers elongate rapidly after initiation of elongation, eventually leading to the deposit of a large amount of cellulose. To reveal features of cotton fiber cells at the fast elongation and the secondary cell wall synthesis stages, we compared the respective transcriptomes and metabolite profiles. Comparative analysis of transcriptomes by cDNA array identified 633 genes that were differentially regulated during fiber development. Principal component analysis (PCA) using expressed genes as variables divided fiber samples into four groups, which are diagnostic of developmental stages. Similar grouping results are also found if we use non-polar or polar metabolites as variables for PCA of developing fibers. Auxin signaling, wall-loosening and lipid metabolism are highly active during fiber elongation, whereas cellulose biosynthesis is predominant and many other metabolic pathways are downregulated at the secondary cell wall synthesis stage. Transcript and metabolite profiles and enzyme activities are consistent in demonstrating a specialization process of cotton fiber development toward cellulose synthesis. These data demonstrate that cotton fiber cell at a certain stage has its own unique feature, and developmental stages of cotton fiber cells can be distinguished by their transcript and metabolite profiles. During the secondary cell wall synthesis stage, metabolic pathways are streamed into cellulose synthesis.
关键词
棉纤维
细胞延长
细胞壁合成
基因表达
代谢产物
cotton fiber, transcriptome, metabolite profile, auxin, cell elongation, cellulose synthesis