十二烷基苯磺酸钠增敏乙基紫共振光散射法测定人尿中痕量1-羟基芘

Determination of Trace 1-Hydroxypyrene in Human Urine with Ethyl Violet by Resonance Light Scattering Method Using Sodium Dodecylbenzene Sulfonate as Sensitizer

基于乙基紫（EV）与1-羟基芘（1-OHP）反应形成离子缔合物,导致体系的共振光散射（RLS）增强,建立了测定1-OHP的共振光散射法.在pH 8.0Tris-HCl缓冲介质中,最大RLS峰位于396 nm,其强度与1-OHP浓度成线性关系.加入表面活性剂十二烷基苯磺酸钠（SDBS）,体系的灵敏度可提高两倍以上.方法的线性范围为4.0～982 μg·L^-1,相关系数r=0.999 4,检出限1.2 μg·L^-1,相对标准偏差为5.8%～7.9%,平均回收率为94%（n=6）.已成功用于人尿中痕量1-OHP的测定,结果满意.

A novel method for the trace determination of 1-hydroxypyrene （1-OHP） has been developed. It was based on the enhancement of resonance light scattering（RLS） of the ion-associate complex formed between ethyl violet（EV） and 1-OHP. In Tris- HCl buffer solution of pH 8.0, the maximum RLS peak was located at 396 nm and the enhanced RLS intensity at this wavelength was directly proportional to the 1-OHP concentration. The sensitivity of 1-OHP -EV complex in the presence of sodium dodecylbenzene sulfonate （SDBS） was twice as high as that in the absence of surfactant. The linear range of the calibration curve was 4.0 - 982 μg·L^-1 with a correlation coefficient of 0. 999 4 and the detection limit for 1-OHP was 1.2 μg·L^-1. The relative standard deviation was 5.8% -7.9% and the average recovery was 94% （n = 6）. The proposed method has been used for the analysis of 1-OHP in human urine with satisfactory results.