基于聚硫堇和纳米金共修饰的过氧化氢生物传感器的研究

Hydrogen Peroxide Biosensor Based on Poly(thionine) and Au Colloid

用循环伏安法将电子媒介体硫堇电聚合在铂电极上，使其表面形成均匀的带负电的聚合膜层，通过静电吸附作用固定表面带正电荷的辣根过氧化物酶，接着吸附纳米金，然后再利用纳米金吸附固定一层辣根过氧化物酶，制成了新型过氧化氢生物传感器。实验发现，该传感器增加了酶的吸附量，响应快、灵敏度高、稳定性好，对H2O2表现出良好的响应特性。检测范围为5．2×10^-7～2.0×10^-3moL／L，检出限为1，7×10^-7mol／L，并具有抗尿酸、抗坏血酸等干扰的特点。

A novel method for the preparation of a hydrogen peroxide biosensor was described, Thionine was electropolymerized on the platinum electrode to form a negatively charged surface by cyclic voltammetry. Then horseradish peroxidase （HRP）/nano-Au/HRP were immobilized on the electrode modified by poly （thionine） via electrostatic adsorption to prepare the hydrogen peroxide biosensor, Experimental results showed that the biosensor exhibited fast response, high sensitivity and good stability, The linear response of the sensor to hydrogen peroxide was in the concentration range of 5, 2 × 10^-7 - 2.0 × 10^-3 mol/L with a detection limit of 1. 7 × 10 ^-7 mol/L. In addition, the biosensor operated at low potential would be interference-free from ascorbic acid and uric acid,