低能量氦氖激光血管内照射对缺血再灌注心肌超微结构的影响

Effect of low energy He-Ne laser intravascular irradiation on ultrastructure of ischemic reperfusion myocardium

目的:探讨低能量氦氖激光血管内照射(ILIB)对缺血再灌注心肌组织超微结构的影响。方法:通过结扎冠状动脉阻断冠状动脉血流,复制家兔急性心肌缺血-再灌注损伤动物模型。将家兔随机分为4组:假手术组、心肌缺血30min组、缺血30min再灌注30min组、缺血30min再灌注30min+ILIB组。在透射电镜下观察各组心肌超微结构的变化。结果:假手术组心肌纤维呈阶段性收缩、间质水肿、线粒体轻度肿胀、间质可见闭塞的毛细血管;心肌缺血组心肌纤维排列紊乱、肌浆水肿、线粒体水肿、血管扩张、血管结构破坏、血管内可见白细胞镶嵌;缺血再灌注组心肌纤维排列相对整齐、线粒体水肿不明显、肌浆水肿较轻、心肌间质可见轻度毛细血管扩张淤血;缺血再灌注+ILIB组心肌纤维排列整齐、线粒体无肿胀、间质毛细血管内可见红细胞、无毛细血管内白细胞镶嵌现象、毛细血管管腔通畅、血管壁较完整。结论:IL-IB可使缺血再灌注心肌超微结构基本恢复正常,对心肌有保护作用。

Objective： To explore the effect of low energy He-Ne laser intravascular irradiation on ultrastructure of ischemic reperfusion myocardium. Methods： The animal model of acute ischemic reperfusion injury was replicated by ligating coronary artery to obstruct the bloodstream in house rabbit. The rabbits were divided into sham operation, myocardial ischemic for 30 minutes, ischemic and reperfusion separately for 30 minutes and ischemic and reperfusion for 30 minutes with ILIB treatment groups. The changes of myocardial ultrastructure were observed by transmission electron microscope. Results： In sham opera- tion group myocardial myofiber showed stage contraction, interstitial edema, venial chondriosome turgescence, occlusive capillaries; in myocardial ischemia group, myocardial myofiber showed arrangement disturbance, myoplasm edema, chondriosome edema, angiectasis, angiolysis, white blood cell mosaicism in vascular; in ischemic and reperfusion group myocardial myofiber arranged relatively regularly, venial chondriosome edema, venial myoplasm edema, venial interstitial angiotelectasis and congestion; and in vascular ischemic reperfusion with ILIB treatment group myocardial myofiber arranged regularly, no chondriosome edema, erythrocytes in interstitial capillary, fluent capillary lumen,intact vascular wall without white blood cell mosaicism. Conclusion：ILIB treatment can regain ultrastructure of ischemic reperfusion myocardium and so protect the myocardium from the injury.