NF-κB、PKC和PC-PLC在双歧杆菌的脂磷壁酸激活巨噬细胞产生NO中的作用

The role of NF-κB,PKC and PC-PLC on lipoteichoic acid of Bifidobacteria activating macrophages to produce NO

目的探索双歧杆菌的LTA激活巨噬细胞产生一氧化氮(NO)的信号途径。方法以LTA刺激大鼠腹腔巨噬细胞,用激光共聚焦显微镜定量测定其诱导型一氧化氮合酶(iNOS)的含量,以Griess试剂检测巨噬细胞产生NO的含量。结果LTA刺激组大鼠腹腔巨噬细胞iNOS和NO的含量明显高于对照组(P<0.01)。以PDTC、Chelerythrine和D609分别预先孵育巨噬细胞,再以LTA刺激巨噬细胞,其产生iNOS和NO的量明显低于LTA刺激组(P<0.01)。结论双歧杆菌的LTA可通过NF-κB、PKC和PC-PLC激活巨噬细胞,使之产生多量的iNOS以及NO。

Objective To explore the signal pathway of hpoteichoic acid（LTA） of bifidobacteria activating macrophages to produce nitric oxide（NO）. Methods After LTA stimulated mouse peritoneal macrophages ,the content of inducible nitric oxide synthase（iNOS） and NO were detected by using laser confocal microscope and Griess reagent respectively. Results The content of iNOS and NO of mouse peritoneal macrophages in LTA injection group were significantly higher ＇than that in control group（ P 〈0.01 ）. After the macrophages were pretreated with PDTC ,Chelerythrine and D609 respectively, then the macrophages were stimulated by LTA, the content of iNOS and NO produced by the macrophages were obviously lower when compared with LTA stimulating group （ P 〈0.01 ）. Conclusion LTA of bifidobacteria could activate macrophages to produce large amount of iNOS and NO by through NF-κB, PKC and PC-PLC.