产木聚糖酶黑曲霉LN0601的液体发酵

Submerged Fermentation of Aspergillus Niger LN0601 to Produce Xylanase

利用黑曲霉LN0601菌株液体发酵生产木聚糖酶。分别考察培养基碳源、培养基氮源、培养时间、培养温度、培养起始pH及孢子悬液接种体积分数等因素对该菌株产木聚糖酶性能的影响。确立了黑曲霉LN0601产木聚糖酶的最优条件,即以质量分数为5%的玉米芯粉作为培养基碳源;质量分数为1%的NaNO3作为培养基氮源;培养时间为2 d;培养温度为28℃;培养起始pH为6.5;孢子悬液接种量体积分数为20%,接入装有100 mL液体发酵培养基的250 mL三角瓶内,150 r/min振荡培养。此条件下黑曲霉LN0601的木聚糖酶活力可达2 000 U/mL以上。

The Aspergillus niger LN0601 was used to produce xylanase by submerged fermentation. The influence factors of carbon source, nitrogen source, cultural time, cultural temperature, initial pH, and inoculation volumic fraction of spore suspending liquid on the capability of the stain to produce xylanase were studied. The optimum conditions of Aspergillus niger to produce xylanase are as follows： carbon source 5 % （mass fraction） corncob powder, nitrogen source 1 % （mass fraction） NaNO3, cultural time 2 d, cultural temperature 28 ℃, initial pH 6.5, 20% inoculation volumic fraction of spore suspending liquid added to 250 mL conical flask with 100 mL submerged culture medium, shaking table revolution 150 r/min. Under this condition the xylanase activity could reach to more than 2 000 U/mL.