人体血清中原卟啉Ⅸ自体荧光光谱研究

Study of Autofluorescence Spectra of Protoporphyrin Ⅸ in Human Serum

通过对人体血清样品和模拟人体生理条件下原卟啉Ⅸ和牛血清白蛋白混合溶液的荧光光谱测定和分析,表明人体血清中原卟啉Ⅸ的自体荧光光谱主要来自于原卟啉Ⅸ和血清白蛋白的结合形式。此外,还考察了血清白蛋白和原卟啉Ⅸ对原卟啉Ⅸ荧光发射峰的影响。和不含有白蛋白的原卟啉Ⅸ溶液相比较,白蛋白不但使原卟啉Ⅸ产生的荧光峰发生红移,而且具有很强的增敏效应。在白蛋白存在条件下,当原卟啉Ⅸ浓度值小于0.8×10-5mol·L-1时,随着它的浓度增加,原卟啉Ⅸ的荧光发射峰稍有红移;而当原卟啉Ⅸ浓度值大于0.8×10-5mol·L-1时,其荧光发射波长几乎不随原卟啉Ⅸ浓度而变化。

The fluorescence spectra of protoporphyrin Ⅸ （PPⅨ） solutions and human serum samples were measured and analyzed under physiological conditions. The experimental results showed that the fluorescence of PP Ⅸ in human serum was mainly derived from PPⅨ-serum albumin complex. Moreover, the effects of serum albumin and PPⅨ on the PP Ⅸ emission fluorescence were also investigated. Compared with pure PPⅨ solution, not only a red-shift of PPⅨ fluorescence peak was found for PPⅨ ＋albumin solution, but also albumin had a fluorescence enhancement effect on protoporphyrin Ⅸ. For the mixture of PPⅨ ＋albumin, with the increase of PPⅨ the wavelength of the PPⅨ emission peak will increased a little when its concentration was less than 0. 8×10^-5 mol·L^-1 , but was nearly invariable when its concentration was more than 0. 8×10^-5 mol·L^-1.